How to reconstitute
Lyophilized peptides are a dry cake. Bacteriostatic water turns that cake into a solution you can measure. The calculator does the arithmetic; this page is the bench method behind every sheet.
- 1
Let the vial warm
A freezer or fridge vial sweats if you open it cold. Sit it 15–30 minutes until the glass is near room temperature, then wipe the stopper with alcohol and let it dry. Do not skip the wait — condensate is how cakes collapse.
- 2
Keep the diluent at room temperature
Bacteriostatic water stays at 20–25 °C. Do not refrigerate the unopened diluent. More water means a larger, easier draw for a small amount. Less water keeps a large weekly amount on a 1 mL syringe. Each protocol page suggests a starting volume for the catalog vial size.
- 3
Introduce water down the glass
Draw bacteriostatic water, insert the needle through the stopper, and let the stream run down the inside wall. Do not blast the cake.
- 4
Swirl until clear
Roll the vial between your palms. Do not shake. Foam and bubbles mean you were too vigorous. If particles remain after a few minutes, discard.
- 5
Label the math and box it
Write the date, the milligrams in the vial, the millilitres added, and the resulting mg/mL. Mixed vials stay in their box, dark, at 2–8 °C.
2–8 °C, dark, up to 45 days when mixed with bacteriostatic water. Mixed vials stay in their box. Sterile water (no preservative) is single-use. Bacteriostatic water: 20–25 °C. Do not refrigerate the diluent. Sterile water without preservative is single-use. Glutathione is the thiol exception on this site — use it promptly even if the molecule’s safety file is otherwise quiet. Amounts on the protocol sheets are reconstitution math, not a recommendation.