Growth hormone · GnRH-axis research; fertility and HSDD studies
Kisspeptin-10
KISS1 45-54, Metastin 45-54, KP-10
A decapeptide ligand of GPR54 (KISS1R) on hypothalamic GnRH neurons. The focus is HPG-axis regulation — LH/FSH downstream — plus anti-angiogenic/metastin history. Literature score 75/100.
- GnRH/LH/FSH pulse
- Reproductive axis
- Catalog fill
- 10 mg
- Typical water
- 2 mL
- Route
- Subcutaneous
- Cycle
- Short research blocks of 2–4 weeks.
- Approval
- Phase 2 territory, not an approved fertility drug on this page.
- Status
- Research chemical. Some human experimental work.
- Evidence
- Human studies in HSDD and fertility; generally well tolerated in those files.
- Research score
- 75 / 100
Mechanism
GPR54 on GnRH neurons, Gq/11, calcium and PLC, then a GnRH pulse and pituitary LH/FSH.
Safety file
Favourable in the human studies cited. Short half-life. Still not a fertility protocol.
- Hormone fluctuation
- Injection-site reactions
- Headache
- Cycle timing change
Reconstitution
2 mL bacteriostatic water into 10 mg gives 5 mg/mL, 50 mcg/unit. A 100 mcg pulse is 2 units.
Research amounts
A few times per week, not necessarily daily.
| Step | Amount | Frequency | Notes |
|---|---|---|---|
| Common pulse | 100 mcg | Several times weekly | 2 units from a 2 mL reconstitution |
| Upper cited pulse | 200 mcg | Several times weekly | 4 units |
Storage
- Lyophilized
- −20 °C freezer preferred; a refrigerator is also acceptable. Do not open a cold vial — wait 15–30 minutes so it does not sweat. Avoid freeze–thaw cycling.
- After water
- 2–8 °C, dark, up to 45 days when mixed with bacteriostatic water. Mixed vials stay in their box. Sterile water (no preservative) is single-use.
Worksheet notes
- This worksheet is not a fertility protocol and does not replace endocrine care.
- Bacteriostatic water: 20–25 °C. Do not refrigerate the diluent.
- Amounts on this sheet are reconstitution math for the vial, not a recommendation.
Full protocol
Titration, schedule, and handling
The complete protocol for Kisspeptin-10. Where it differs from the worksheet amounts above, the worksheet reflects the NovaEvum vial.
Reconstitution
| Vial | 2 mL water | 3 mL water |
|---|---|---|
| 10 mg | 5 mg/mL | 3.33 mg/mL |
- Use bacteriostatic water as the diluent.
- Add the water slowly, letting it run down the inside wall of the vial rather than onto the powder.
- Swirl gently to mix, then leave it to dissolve. Do not shake.
- Give it a few minutes. The finished solution should be clear and colourless.
Protocol
Route: Subcutaneous
Community protocol (subcutaneous)
- A commonly used range is 0.1 mg to 0.2 mg (100 to 200 mcg) per dose, used in short courses rather than continuously
- Some run it alongside TRT support to nudge the natural hormone axis
Honest note on the data
- Most published human kisspeptin research uses intravenous infusions and the longer kisspeptin-54, not subcutaneous kisspeptin-10, so a precise injectable protocol is not well established
- Treat the above as a conservative community starting point and assess carefully
Dosing detail
- Community subcutaneous dosing is commonly 0.1 mg to 0.2 mg per dose, in short courses.
- Acts upstream on the hormone axis to stimulate LH and downstream testosterone.
- Strong human data is mostly from IV infusion studies, so injectable protocols are less settled than for, say, the GLP-1 drugs.
- Generally well tolerated in studies.
Duration
- Typically used in shorter cycles rather than continuously.
Notes
- Acts upstream on the hormone axis to stimulate LH and testosterone.
- Inject subcutaneously and rotate sites.
- Generally well tolerated.
Storage and handling
- Keep the powdered (lyophilized) vial in a cool, dark place. Refrigerate it for longer-term storage before mixing.
- Once reconstituted and refrigerated at 2–8 °C away from light, use the vial within 90 days.
- Do not freeze a reconstituted vial, and keep it away from heat and sunlight.
- If the solution turns cloudy, develops floating particles, or shows any other sign of degradation, stop using that vial.