Longevity & cellular · Cellular NAD+ pool; ageing and metabolic research

NAD+

NAD+, NMN, NR, Coenzyme I

NAD+ is usually studied alongside its precursors NMN and NR. Oral NR is GRAS. The NovaEvum vial is the cofactor itself, not a capsule. Levels fall with age; the literature is mostly precursor supplementation, not subcutaneous NAD+ clinic protocols.

Library entry →

Mitochondrial function
Sirtuins
DNA repair (mechanistic)

Catalog fill: 500 mg
Typical water: 5 mL
Route: Subcutaneous (slow) or as specified by the protocol under study
Cycle: 4–8 weeks.
Approval: GRAS / dietary-supplement path for NR. Injectable NAD+ is a research chemical on this catalog.
Status: NR has GRAS as a supplement. Injectable NAD+ is not that oral file.
Evidence: NMN/NR in trials for ageing, metabolic health, neurodegeneration.
Research score: 85 / 100

Mechanism

NMN/NR enter the salvage path to NAD+. NAD+ fuels redox metabolism and sirtuins (SIRT1/SIRT3). Direct NAD+ skips that oral conversion.

Safety file

Oral precursors: mild GI, flushing. Short-term studies look quiet. Injectable sting and longer-term data are a different question.

Nausea
Headache
Flushing
Sleep change
Fatigue

Reconstitution

5 mL bacteriostatic water into 500 mg gives 100 mg/mL. A 50 mg amount is 50 units (0.5 mL). A 1000 mg vial at the same concentration takes 10 mL.

Research amounts

1–3 times weekly in many research worksheets; some run smaller daily amounts.

Step	Amount	Frequency	Notes
Common subcutaneous amount	50 mg	1–3× weekly	50 units at 100 mg/mL
Upper cited amount	100 mg	1–2× weekly	100 units

Storage

Lyophilized: −20 °C freezer preferred; a refrigerator is also acceptable. Do not open a cold vial — wait 15–30 minutes so it does not sweat. Avoid freeze–thaw cycling.
After water: 2–8 °C, dark, up to 45 days when mixed with bacteriostatic water. Mixed vials stay in their box. Sterile water (no preservative) is single-use.

Worksheet notes

Subcutaneous NAD+ can sting. A slower push and a more dilute reconstitution are the usual adjustments.
This is not IV clinic protocol and does not describe infusion.
Bacteriostatic water: 20–25 °C. Do not refrigerate the diluent.
Amounts on this sheet are reconstitution math for the vial, not a recommendation.

Full protocol

Titration, schedule, and handling

The complete protocol for NAD+. Where it differs from the worksheet amounts above, the worksheet reflects the NovaEvum vial.

Reconstitution

Concentration by vial size and water volume
Vial	5 mL water	10 mL water
500 mg	100 mg/mL	50 mg/mL

NAD+ is quite acidic and can sting or burn going in. A larger fill (10 mL) and a slow push both reduce that. Subcutaneous doses are usually small and ramped up gradually.

Use bacteriostatic water as the diluent.
Add the water slowly, letting it run down the inside wall of the vial rather than onto the powder.
Swirl gently to mix, then leave it to dissolve. Do not shake.
Give it a few minutes. The finished solution should be clear and colourless.
Because it is acidic, plan to inject slowly to limit the sting.

Protocol

Route: Subcutaneous

Standard protocol (subcutaneous)

Week 1: start at 20 mg per dose
Week 2: 50 mg
Week 3 onward: 100 mg per dose if well tolerated
Dose 3 times per week (for example Monday, Wednesday, Friday), not daily
At a 10 mL fill (50 mg/mL), 50 mg is a 1 mL draw (100 units) and 100 mg is 2 mL (200 units)

Go slow on the plunger

NAD+ is acidic and the burn comes from injecting too fast
Push the dose over a full 30 to 60 seconds
The bigger 10 mL fill also keeps it more dilute and easier to tolerate

Dosing detail

Ramp over 3 weeks: 20 mg, then 50 mg, then 100 mg per dose, three times per week.
Three sessions a week (e.g. Mon/Wed/Fri) is the common frequency, not daily.
The discomfort is from acidity and injection speed, so push slowly over 30 to 60 seconds.
Studied for cellular energy and longevity.

Duration

Run three times weekly in blocks of 4 to 6 weeks, then assess.

Notes

Studied for cellular energy and longevity.
The sting is from acidity, so dilute more and go slow.
Inject subcutaneously and rotate sites.

Storage and handling

Keep the powdered (lyophilized) vial in a cool, dark place. Refrigerate it for longer-term storage before mixing.
Once reconstituted and refrigerated at 2–8 °C away from light, use the vial within 90 days.
Do not freeze a reconstituted vial, and keep it away from heat and sunlight.
If the solution turns cloudy, develops floating particles, or shows any other sign of degradation, stop using that vial.