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GLP-1 & metabolic · Type 2 diabetes and obesity management

Semaglutide

0.25 → 2.4 mg weekly, titrated

A long-acting GLP-1 receptor agonist approved as Ozempic and oral Rybelsus for type 2 diabetes and as Wegovy for weight management. It has driven major gains in obesity treatment, though GI side effects require gradual dose increases.

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Reconstitution

Concentration by vial size and water volume
Vial1 mL water2 mL water
5 mg5 mg/mL2.5 mg/mL
10 mg10 mg/mL5 mg/mL

Dosed in small amounts, so a 2 mL fill on a 5 mg vial (2.5 mg/mL) keeps the early micro-doses measurable. Adjust the fill to suit the dose you are running.

  1. Use bacteriostatic water as the diluent.
  2. Add the water slowly, letting it run down the inside wall of the vial rather than onto the powder.
  3. Swirl gently to mix, then leave it to dissolve. Do not shake.
  4. Give it a few minutes. The finished solution should be clear and colourless.

Protocol

Route: Subcutaneous

Standard titration (once weekly, subcutaneous)

  • Weeks 1 to 4: 0.25 mg
  • Weeks 5 to 8: 0.5 mg
  • Weeks 9 to 12: 1 mg
  • Weeks 13 to 16: 1.7 mg
  • Week 17 onward: 2.4 mg as the target maintenance dose
  • Hold each step a full 4 weeks

If 2.4 mg is too much

  • 1.7 mg is a recognised fallback maintenance dose
  • If nausea at 2.4 mg does not settle after a few weeks, dropping back to 1.7 mg is normal and still effective

Dosing detail

  • Start at 0.25 mg for 4 weeks. This is a tolerance-building dose and is not expected to do much for weight on its own.
  • Step up every 4 weeks: 0.25, 0.5, 1.0, 1.7, 2.4 mg, reaching target around week 16.
  • 2.4 mg is the standard maintenance dose for weight, with 1.7 mg as a fallback if 2.4 is not tolerated.
  • Same day each week. Half-life is about 7 days, so timing is forgiving.

Duration

  • Long-run protocol. Titrate over the first ~16 weeks, then maintain. Stopping commonly leads to weight regain, so plan an exit (taper or maintenance dose) with that in mind.

Notes

  • Inject subcutaneously, rotating sites.
  • Nausea is the most reported early effect and a smaller starting dose helps.
  • Protein and hydration matter as appetite falls.

Storage and handling

  • Keep the powdered (lyophilized) vial in a cool, dark place. Refrigerate it for longer-term storage before mixing.
  • Once reconstituted and refrigerated at 2–8 °C away from light, use the vial within 90 days.
  • Do not freeze a reconstituted vial, and keep it away from heat and sunlight.
  • If the solution turns cloudy, develops floating particles, or shows any other sign of degradation, stop using that vial.