Longevity & cellular · Endogenous antioxidant and detoxification tripeptide
Glutathione
GSH, Reduced glutathione, γ-L-glutamyl-L-cysteinyl-glycine
The body’s thiol tripeptide (glutamate–cysteine–glycine with a gamma-glutamyl bond). Often called the master antioxidant, score 85/100, safety ‘excellent’ at ordinary amounts. The gamma bond is why we do not write it as ECG.
- Antioxidant
- Immune
- Detoxification
- Catalog fill
- 1500 mg
- Typical water
- 5 mL
- Route
- Subcutaneous or as specified by the protocol under study
- Cycle
- 4–8 weeks.
- Approval
- Multiple approved contexts exist for glutathione as a substance; this catalog vial is still a research fill.
- Status
- OTC in many oral/topical forms. Parenteral use is a different product class.
- Evidence
- Extensive human literature as a molecule.
- Research score
- 85 / 100
Mechanism
GSH/GSSG redox cycle. The cysteine thiol scavenges radicals, recycles vitamins C and E, and feeds conjugation/detox paths.
Safety file
Generally well tolerated at standard amounts. Reconstituted thiols still oxidise in the vial — colour change means discard.
- Usually quiet at ordinary amounts
Reconstitution
The catalog fill is 1500 mg. 5 mL bacteriostatic water yields 300 mg/mL. A 200 mg amount is 0.67 mL (67 units); 600 mg is 2 mL.
Research amounts
Several times weekly in many worksheets.
| Step | Amount | Frequency | Notes |
|---|---|---|---|
| Lower research amount | 200 mg | Several times weekly | 67 units at 300 mg/mL |
| Upper cited amount | 600 mg | Several times weekly | 200 units — use more water if you need the draw under 1 mL |
Storage
- Lyophilized
- −20 °C freezer preferred; a refrigerator is also acceptable. Do not open a cold vial — wait 15–30 minutes so it does not sweat. Avoid freeze–thaw cycling.
- After water
- 2–8 °C, dark. Use promptly once mixed — thiols oxidise even though the molecule's own safety profile is excellent.
Worksheet notes
- The gamma-glutamyl bond is not a standard alpha-peptide sequence. Do not write it as ECG.
- Discoloration after reconstitution means the thiol has oxidized — discard.
- Bacteriostatic water: 20–25 °C. Do not refrigerate the diluent.
- Amounts on this sheet are reconstitution math for the vial, not a recommendation.
Full protocol
Titration, schedule, and handling
The complete protocol for Glutathione. Where it differs from the worksheet amounts above, the worksheet reflects the NovaEvum vial.
Reconstitution
| Vial | 3 mL water | 5 mL water | 10 mL water |
|---|---|---|---|
| 1500 mg | 500 mg/mL | 300 mg/mL | 150 mg/mL |
A large 1500 mg vial. Filling to 5 mL gives 300 mg/mL, which keeps common doses on a standard 1 mL insulin syringe: 200 mg is 67 units, 300 mg a full 100 units. A 3 mL fill (500 mg/mL) makes draws smaller still (200 mg is 40 units). For larger single doses like 600 mg, split into two insulin-syringe injections at different sites, or fill to 10 mL and use a 3 mL syringe with a higher-gauge needle, where 600 mg is a 4 mL draw.
- Use bacteriostatic water as the diluent.
- Add the water slowly, letting it run down the inside wall of the vial rather than onto the powder.
- Swirl gently to mix, then leave it to dissolve. Do not shake.
- Give it a few minutes. The finished solution should be clear and colourless.
Protocol
Route: Subcutaneous or intramuscular
Standard protocol (subcutaneous or intramuscular)
- 200 mg to 600 mg per injection, 2 to 3 times weekly is the common range for antioxidant and detox support
- Skin-brightening protocols run at the higher end
- At a 5 mL fill on the 1500 mg vial (300 mg/mL), 200 mg is a 0.67 mL draw (67 units) and 300 mg is a full 1 mL (100 units) on an insulin syringe
- A 600 mg dose is 2 mL, more than one insulin syringe holds, so split it into two injections or use a 3 mL syringe
- Injecting more than about 1.5 mL into a single subcutaneous site tends to leak, so splitting a large dose across two sites is good practice anyway
Timing and pairing
- Morning on an empty stomach is a common choice
- Do not mix it in the same syringe as vitamin C; they can react and cloud the solution, so inject them separately if stacking
- Some pair it with oral NAC to support the body's own glutathione production
Dosing detail
- Common dose is 200 mg to 600 mg per injection, 2 to 3 times weekly. Higher end for skin brightening.
- For 600 mg or larger, split into two insulin-syringe injections, or fill to 10 mL and use a 3 mL syringe with a higher-gauge needle.
- Inject subcutaneously or intramuscularly; morning on an empty stomach is common. Rotate sites.
- Do not combine in the same syringe as vitamin C, as they can react. Inject separately if stacking.
Duration
- Load 2 to 3 times weekly for 4 to 8 weeks, then once weekly to maintain. Since the body makes glutathione naturally, continuous use is possible, but a 12 weeks on, 4 weeks off pattern is common.
Notes
- The body's master antioxidant, a tripeptide of glutamate, cysteine and glycine. Injected because oral glutathione is poorly absorbed.
- Used for antioxidant support, liver and detox pathways, and off-label for skin brightening.
- Well tolerated. Inject slowly and rotate sites.
Storage and handling
- Keep the powdered (lyophilized) vial in a cool, dark place. Refrigerate it for longer-term storage before mixing.
- Once reconstituted and refrigerated at 2–8 °C away from light, use the vial within 90 days.
- Do not freeze a reconstituted vial, and keep it away from heat and sunlight.
- If the solution turns cloudy, develops floating particles, or shows any other sign of degradation, stop using that vial.