Healing & recovery · Immune modulation; viral infection protocols outside the US
Thymosin Alpha-1
Zadaxin, Thymalfasin, TA1, Tα1
A 28-residue thymic peptide (thymalfasin / Zadaxin). The literature rates 90/100: an immunomodulator used outside the US for viral hepatitis and as an adjuvant, with a generally quiet adverse-event profile.
- Immune
- Antiviral
- Anti-inflammatory
- Catalog fill
- 10 mg
- Typical water
- 2 mL
- Route
- Subcutaneous
- Cycle
- 4–8 weeks.
- Approval
- Approved in many countries for hepatitis B/C and as a vaccine adjuvant; not a US catch-all.
- Status
- Prescription in some countries. Not a US FDA approval for the indications discussed.
- Evidence
- Large trial safety file. Recent interest in COVID/PASC and oncology adjuvants.
- Research score
- 90 / 100
Mechanism
T-cell maturation via TLR2/TLR9, Th1 cytokines (IL-2, IFN-γ, IL-12), MHC I, dendritic-cell maturation, NK activity.
Safety file
Adverse events are rare and usually local. Large trials have not shown significant drug-related toxicity.
- Injection-site reactions
- Mild fatigue
- Short flu-like symptoms
Reconstitution
2 mL bacteriostatic water yields 5 mg/mL. A 1.6 mg draw is 32 units.
Research amounts
Twice weekly, at least 72 hours apart.
| Step | Amount | Frequency | Notes |
|---|---|---|---|
| Common research amount | 1.5–1.6 mg | Twice weekly | 30–32 units from a 2 mL reconstitution |
Storage
- Lyophilized
- −20 °C freezer preferred; a refrigerator is also acceptable. Do not open a cold vial — wait 15–30 minutes so it does not sweat. Avoid freeze–thaw cycling.
- After water
- 2–8 °C, dark, up to 45 days when mixed with bacteriostatic water. Mixed vials stay in their box. Sterile water (no preservative) is single-use.
Worksheet notes
- Do not confuse with TB-500 (thymosin beta-4 fragment). The two are different molecules and different amounts.
- Bacteriostatic water: 20–25 °C. Do not refrigerate the diluent.
- Amounts on this sheet are reconstitution math for the vial, not a recommendation.
Full protocol
Titration, schedule, and handling
The complete protocol for Thymosin Alpha-1. Where it differs from the worksheet amounts above, the worksheet reflects the NovaEvum vial.
Reconstitution
| Vial | 2 mL water | 3 mL water |
|---|---|---|
| 10 mg | 5 mg/mL | 3.33 mg/mL |
- Use bacteriostatic water as the diluent.
- Add the water slowly, letting it run down the inside wall of the vial rather than onto the powder.
- Swirl gently to mix, then leave it to dissolve. Do not shake.
- Give it a few minutes. The finished solution should be clear and colourless.
Protocol
Route: Subcutaneous
Standard protocol (subcutaneous)
- 1.5 mg per dose (clinical studies use 1.6 mg)
- For general immune support, twice weekly
- When actively fighting something off, a short course of daily or twice-daily dosing is used
- At a 2 mL fill on a 10 mg vial (5 mg/mL), 1.5 mg is a 0.3 mL draw (30 units)
Course example
- The sepsis trial model was 1.6 mg twice daily for 5 days, then once daily for 2 days
- For everyday immune support, the lighter twice-weekly approach is more typical
Dosing detail
- Common dose is 1.5 mg (clinical 1.6 mg) per injection.
- Maintenance immune support: twice weekly. Acute short course: daily or twice daily for several days.
- An immune-modulating peptide.
- Generally well tolerated.
Duration
- Twice weekly for ongoing support, or a short daily course (around a week) when acutely needed.
Notes
- An immune-modulating peptide.
- Generally well tolerated.
- Inject subcutaneously and rotate sites.
Storage and handling
- Keep the powdered (lyophilized) vial in a cool, dark place. Refrigerate it for longer-term storage before mixing.
- Once reconstituted and refrigerated at 2–8 °C away from light, use the vial within 90 days.
- Do not freeze a reconstituted vial, and keep it away from heat and sunlight.
- If the solution turns cloudy, develops floating particles, or shows any other sign of degradation, stop using that vial.