Healing & recovery · Tissue repair and cardiovascular protection

TB-500

Tβ4 fragment, T-beta-4, Ac-SDKP

A synthetic fragment of thymosin beta-4 (often residues 17–23), sold separately from the full-length protein that is actually in wound-healing and cardiac trials. TB-500 is a research chemical with no formal human safety file.

Library entry →

Healing
Angiogenesis
Anti-inflammatory
Wound healing

Catalog fill: 10 mg
Typical water: 2 mL
Route: Subcutaneous
Cycle: 4-week load, 4–6 week maintenance, then stop and reassess.
Approval: Research chemical only. Full-length thymosin beta-4 is in trials; the TB-500 fragment is not.
Status: Research chemical. WADA banned.
Evidence: Full-length Tβ4 has trials. The sold fragment has none.
Research score: 60 / 100

Mechanism

G-actin sequestration and cell migration, plus VEGF/bFGF. The Ac-SDKP fragment is the piece tied to TGF-β and fibrosis in cardiac models.

Safety file

Full-length Tβ4 looks safe in trials. TB-500’s human safety is unknown. Theoretical cancer risk. WADA prohibited.

Joint pain
Headache
Fatigue
Temporary local hair growth

Reconstitution

2 mL bacteriostatic water into a 10 mg vial gives 5 mg/mL. A 2 mg draw is 40 units on a U-100 syringe.

Research amounts

Twice weekly for 4 weeks (loading), then once weekly.

Step	Amount	Frequency	Notes
Loading	2.0–2.5 mg	Twice weekly	40–50 units from a 2 mL reconstitution
Maintenance	2.0 mg	Once weekly	40 units

Storage

Lyophilized: −20 °C freezer preferred; a refrigerator is also acceptable. Do not open a cold vial — wait 15–30 minutes so it does not sweat. Avoid freeze–thaw cycling.
After water: 2–8 °C, dark, up to 45 days when mixed with bacteriostatic water. Mixed vials stay in their box. Sterile water (no preservative) is single-use.

Worksheet notes

Amounts are milligrams, not micrograms — a 2 mg draw is eight times a 250 mcg BPC-157 draw.
Often paired with BPC-157 rather than run alone. See Wolverine Stack if both are in one vial.
Bacteriostatic water: 20–25 °C. Do not refrigerate the diluent.
Amounts on this sheet are reconstitution math for the vial, not a recommendation.

Full protocol

Titration, schedule, and handling

The complete protocol for TB-500. Where it differs from the worksheet amounts above, the worksheet reflects the NovaEvum vial.

Reconstitution

Concentration by vial size and water volume
Vial	2 mL water	3 mL water
10 mg	5 mg/mL	3.33 mg/mL

Use bacteriostatic water as the diluent.
Add the water slowly, letting it run down the inside wall of the vial rather than onto the powder.
Swirl gently to mix, then leave it to dissolve. Do not shake.
Give it a few minutes. The finished solution should be clear and colourless.

Protocol

Route: Subcutaneous

Standard protocol (subcutaneous)

Loading: 2 mg to 2.5 mg twice weekly (spaced 3 to 4 days apart, e.g. Monday and Thursday) for 4 to 6 weeks
Maintenance: 2 mg once weekly after the loading phase

Where it goes

TB-500 works systemically, so the injection site does not need to be near the injury
Rotate sites
Often run as the partner to BPC-157 in a healing stack

Dosing detail

Loading: about 2 to 2.5 mg twice weekly for 4 to 6 weeks.
Maintenance: about 2 mg once weekly thereafter.
Long half-life, so twice-weekly dosing is fine; site does not matter since it acts systemically.
TB-500 is on the WADA prohibited list and is banned in competitive sport.

Duration

Load for 4 to 6 weeks, then weekly maintenance. Common overall cycle is 8 to 12 weeks on, then a break.

Notes

The common partner to BPC-157 for recovery stacks.
Well tolerated by most.
Rotate injection sites.

Storage and handling

Keep the powdered (lyophilized) vial in a cool, dark place. Refrigerate it for longer-term storage before mixing.
Once reconstituted and refrigerated at 2–8 °C away from light, use the vial within 90 days.
Do not freeze a reconstituted vial, and keep it away from heat and sunlight.
If the solution turns cloudy, develops floating particles, or shows any other sign of degradation, stop using that vial.