Skin, hair & aesthetics · blend vial · Four-peptide aesthetic/recovery blend
KLOW
GHK-Cu + KPV + TB-500 + BPC-157
NovaEvum 80 mg fill: 50 mg GHK-Cu, 10 mg KPV, 10 mg TB-500, 10 mg BPC-157. The science is those four compounds — copper-peptide skin work, α-MSH fragment inflammation, and two repair fragments.
- Skin
- Healing
- Anti-inflammatory
- Catalog fill
- 80 mg blend
- Typical water
- 4 mL
- Route
- Subcutaneous
- Cycle
- 4–6 weeks.
- Approval
- Sourced from GHK-Cu, KPV, TB-500, and BPC-157 entries.
- Status
- Blend of four research chemicals.
- Evidence
- See the four singles. The 80 mg fill has no independent trial.
- Research score
- 60 / 100
Mechanism
GHK-Cu ECM/copper, KPV cytokine dampening, BPC-157 VEGF/NO, TB-500 actin migration — locked in one ratio.
Safety file
Copper-load and angiogenic caveats from GHK-Cu and BPC-157/TB-500 still apply. Do not add the singles on the same days.
- Injection-site reactions
- Skin irritation
- Headache
Reconstitution
4 mL bacteriostatic water yields 20 mg/mL total. A 0.1 mL (10 unit) draw is 2 mg total peptide: 1.25 mg GHK-Cu and 0.25 mg of each of the other three.
Research amounts
Once daily.
| Step | Amount | Frequency | Notes |
|---|---|---|---|
| Daily research draw | 2 mg total | Once daily | 10 units from a 4 mL reconstitution |
| Smaller daily draw | 1 mg total | Once daily | 5 units |
Storage
- Lyophilized
- −20 °C freezer preferred; a refrigerator is also acceptable. Do not open a cold vial — wait 15–30 minutes so it does not sweat. Avoid freeze–thaw cycling.
- After water
- 2–8 °C, dark, up to 45 days when mixed with bacteriostatic water. Mixed vials stay in their box. Sterile water (no preservative) is single-use.
Worksheet notes
- The certificate of analysis is the record of the actual fill. This worksheet uses the labelled split.
- Do not add extra BPC-157, TB-500, KPV, or GHK-Cu on top without subtracting what KLOW already contributes.
- Bacteriostatic water: 20–25 °C. Do not refrigerate the diluent.
- Amounts on this sheet are reconstitution math for the vial, not a recommendation.
Full protocol
Titration, schedule, and handling
The complete protocol for KLOW. Where it differs from the worksheet amounts above, the worksheet reflects the NovaEvum vial.
Reconstitution
| Vial | 3 mL water | 5 mL water |
|---|---|---|
| 80 mg | 26.67 mg/mL | 16 mg/mL |
KLOW is a blend (GHK-Cu, BPC-157, TB-500, KPV in a 5:1:1:1 ratio), so 80 mg is the combined weight. The vial is usually 3 mL but occasionally ships as 5 mL; use the fill that matches the vial you received. Filling to the top dilutes the GHK-Cu sting as much as the vial allows. The calculator works in total blend mg: since GHK-Cu is 5 of every 8 parts, a 2.5 mg GHK-Cu dose is 4 mg of total blend.
- Use bacteriostatic water as the diluent.
- Add the water slowly, letting it run down the inside wall of the vial rather than onto the powder.
- Swirl gently to mix, then leave it to dissolve. Do not shake.
- Give it a few minutes. The finished solution should be clear and colourless.
- A faint blue tint from the copper is normal.
- The solution should otherwise be clear once mixed.
Protocol
Route: Subcutaneous
How a blend is dosed
- Like GLOW, dose KLOW by the GHK-Cu amount; the BPC-157, TB-500 and KPV follow in the 5:1:1:1 ratio
- The common range is 1 mg to 2.5 mg of GHK-Cu per day, with around 2.5 mg typical during loading
- At a 3 mL fill the GHK-Cu portion comes to about 16.7 mg/mL, so 1 mg of GHK-Cu is a 0.06 mL draw (6 units) and 2.5 mg is 0.15 mL (15 units)
- These figures are for the usual 3 mL fill; with a 5 mL vial, recalculate the draws
Schedule
- One subcutaneous injection daily, or 5 days on, 2 days off, rotating sites
- Run an 8 to 12 week block, then 4 to 8 weeks off
- Inject slowly to limit the GHK-Cu sting
- KLOW adds KPV, so it leans more anti-inflammatory than GLOW
Dosing detail
- Dose by GHK-Cu content, 1 mg to 2.5 mg daily, scaling the rest of the blend with it. Around 2.5 mg is common during loading.
- Once daily, subcutaneous, sites rotated.
- Inject slowly given the copper content.
Duration
- Typically run 8 to 12 weeks, often 5 days on and 2 off (or daily loading then 3 times weekly), then 4 to 8 weeks off and reassess.
Notes
- Adds KPV (an anti-inflammatory tripeptide) to the GLOW base of GHK-Cu, BPC-157 and TB-500.
- Inject subcutaneously and rotate sites.
- Inject slowly given the copper content.
Storage and handling
- Keep the powdered (lyophilized) vial in a cool, dark place. Refrigerate it for longer-term storage before mixing.
- Once reconstituted and refrigerated at 2–8 °C away from light, use the vial within 90 days.
- Do not freeze a reconstituted vial, and keep it away from heat and sunlight.
- If the solution turns cloudy, develops floating particles, or shows any other sign of degradation, stop using that vial.
- A faint blue tint is normal because of the GHK-Cu in the blend, not a sign of degradation.